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Agilent Series Preparative Pump User Manual - Product Details
Solvents containing volatile ingredients will slightly lose these. Therefore priming of the pumping system is required before starting an application. Select 0. Select 1 V to set the full-scale output to 1 volt. Page 54 Negative or Positive. The attenuation settings helps to keep all peaks on scale.
Choose the appropriate setting from the list. Page 57 This should only be used if the content of the reference cell is expected to degrade during a run. The automatic purge will be finished before the autozero is performed and before the injection is done. When required If you want to checkout the detector Hardware required Page 62 Only similar zooming factors in the display of a chromatogram will lead to similar looking results.
Page 65 If you are not using the Agilent ChemStation go to step Page 69 For the Control Module Rescale the plot and measure the baseline noise and drift on the screen. If a printer is configured for the Agilent Series instrument the plot can be printed by pressing the m key and selecting Print Plot.
Page Refractive Index Detector Optimization Confirm that the instrument is free from leaks by performing the Agilent Series diagnostic pressure test for the high pressure parts of the system between pump and column. Ensure that the connections from the on-line vacuum degasser to the pump and the detector inlet, waste and recycle connections are air tight. Page 73 use adapter , which is delivered with the accessory kit of the detector.
Page Potential Causes For Baseline Problems Drift Excessive drift is an indication for a general system or environmental instability system or laboratory might not be thermally stable, control instrument and laboratory temperature. Page Detector Equilibration Switch valves and settings only when needed. Do not expose the detector to draft of air or to vibrations. A change of any of these parameters may require a considerable amount of time for re-equilibration.
Page Status Indicators The power supply indicator is integrated into the main power switch. When the indicator is illuminated green the power is ON. An error condition indicates the detector has detected an internal problem which affects correct operation of the detector. Usually, an error condition requires attention e. An error condition always interrupts the analysis. Page Introduction To The Repairing The Refractive Index Detector The security lever at the power input socket prevents the detector cover from being removed when line power is still connected.
In order to prevent damage, always use an ESD strap supplied in the standard accessory kit of other Agilent Series modules when handling electronic boards and components. Page Detector Maintenance Procedures If flow cell is contaminated. Leak sensor If leak has occurred. Check for leaks. System replacement Replacing the If not up to date or corrupted.
Do not exceed the flow cell pressure limit of 5 bar 0. Page Correcting Leaks 4 Observe the interface ports and the valve area for leaks and correct, if required. Page A Safety Information Make sure that only fuses with the required rated current and of the specified type normal blow, time delay, and so on are used for replacement.
The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Page 93 When working with solvents please observe appropriate safety procedures e. Page 94 A caution alerts you to situations that could cause a possible loss of data. Do not dispose off in domestic household waste To return unwanted products, contact your local Agilent office, or see www. Print page 1 Print document pages.
Rename the bookmark. In addition, it would take a noticeable amount of time to recompress the solvent to operating pressure. During this time frame, no solvent would be delivered into the system and as a result a high pressure fluctuation known as pressure ripple would be observed. When both solvent compressibility at the current operating pressure and pump elasticity are known, the pump can automatically correct for the missing volume by drawing the appropriate larger solvent volume at ambient pressure and speed up the piston during the recompression phase in the first pump chamber.
As a result, the pump delivers the accurate volume with any calibrated solvent at any pressure at a greatly reduced pressure ripple. For applications that require lowest transition volume of the pump, damper and mixer can be bypassed. For compatibility with older methods from GA binary pumps, the old onepoint compressibility compensation is available, too. However, since the compressibility is a non-linear function, one single compressibility value per solvent will only give good results at one particular pressure which is at bar for the GA binary pump.
Incorrect pump elasticity calibration. Solvent compressibility calibrations acquired with a miscalibrated pump will work, but they are not transferable to other pumps.
A correct pump elasticity calibration is an essential prerequisite for successful solvent compressibility calibrations. The two connectors for the CAN bus are used for internal Agilent Series module data transfer and synchronization. This connector needs to be activated by the configuration switch module at the rear of the pump.
The software needs the appropriate drivers to support this communication. See your software documentation for further information. Maximum power consumption is VA Volt-Amps. There is no voltage selector on your Binary Pump SL because the power supply has wide-ranging capability. There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.
The security lever at the power input socket prevents that the Binary Pump SL cover is taken off when line power is still connected.
The vacuum degassers have a special connector for specific use. For details see the degasser manual. For identification and location of the connectors see Figure 6 onpage This chapter provides information about site requirements and specifications for the Binary Pump SL.
Power Consideration The module power supply has wideranging capability see Table 2 on page It accepts any line voltage in the range described in the above mentioned table. Consequently there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result, if the devices are connected to a line voltage higher than specified.
Module is partially energized when switched off, as long as the power cord is plugged in. Repair work at the module can lead to personal injuries, e. Unaccessable power plug. In case of emergency it must be possible to disconnect the instrument from the power line at any time. Power Cords Different power cords are offered as options with the module.
The female end of all power cords is identical. It plugs into the power-input socket at the rear of the module. The male end of each power cord is different and designed to match the wall socket of a particular country or region.
Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit. Bench Space The module dimensions and weight see Table 2 on page 29 allow to place the module on almost any laboratory bench.
It needs an additional 2. If the bench should carry a complete Agilent Series system, make sure that the bench is designed to carry the weight of all the modules. Environment Your pump will work within the specifications at ambient temperature and relative humidity described in Table 2 on page Condensation within the module Condensation will damage the system electronics.
The pump is designed to operate in a typical electromagnetic environment EN where RF transmitters, such as mobile phones, should not be used in close proximity. Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve.
Extensive diagnostics, error detection and display through Agilent LC Diagnostics , leak detection, safe leak handling, leak output signal for shutdown of pumping system.
Low voltages in major maintenance areas. Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre-defined and user-settable limits and feedback messages. Electronic records of maintenance and errors. All specification measurements are done with degassed solvents. Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately.
Inform your service representative that the detector may have been damaged during shipment. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged. Delivery Checklist Compare the delivery checklist with the contents of the shipping boxes to ensure completeness of the shipment. The contents lists is shown in Table 4 on page Please report missing or damaged parts to your local Agilent Technologies sales and service office.
Table 4. Solvent cabinet 4 bottles, for pumps with solvent selection valve , or Solvent cabinet 2 bottles, for pumps without solvent selection valve SB-C18, 2. It is optimized for high flow rates and maximum sensitivity. For a more detailed help on configuring your instrument, refer to the RRLC Configurator part number: The security lever prevents that the cover is opened while the power cord is connected to the pump.
The Agilent Series vacuum degasser is an exception. It can be connected via APG remote connector to the other modules in the stack. Although the LAN board can be installed into all modules except for the degasser and the Thermostatted Column Compartment, the LAN port of the detector should be used as the detector generates the highest data rate of all modules. For more information about connecting the Instant Pilot or Agilent Data System refer to the respective user manual.
The power switch stays pressed in and the green indicator LED in the power switch is on while the pump is turned on. When the line power switch stands out and the green light is off, the pump is turned off.
The pump is shipped with default configuration settings. When opening capillary or tube fittings solvents may leak out. The handling of toxic and hazardous solvents and reagents can hold health risks. Make sure to use the brown bottle for the aqueous solvent usually channel A1. Place the end into your waste system. Installing the Pump Flow Connections without Solvent Selection Valve 4 Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the active inlet valves.
Fix the tubes in the clips of solvent cabinet and Binary Pump SL. Before a new degasser or new solvent tubing can be used, it is necessary to prime the system. Isopropanol IPA is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties.
Connect all modules hydraulically as described in the respective module manuals. Fill each solvent bottle with mL isopropanol Switch the system on. If the pump is not able to aspirate the solvent from the bottles, a syringe can be used to draw the solvent manually through tubing and degasser. When priming the vacuum degasser with a syringe, the solvent is drawn through the degasser tubes very quickly.
The solvent at the degasser outlet will therefore not be fully degassed. Pump for approximately 10 minutes at your desired flow rate before starting an analysis. This will allow the vacuum degasser to properly degas the solvent in the degasser tubes. Reattach the tubing to the solvent selection valve. When the pumping system has been turned off for a certain time for example, overnight air will rediffuse into the solvent channel between the vacuum degasser and the pump.
Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time. When the solvent of a channel is to be replaced by another solvent that is not compatible solvents are immiscible or one solvent contains a buffer it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.
Remove the column and replace it by a ZDV fitting. Prepare bottles with appropriate intermediate solvents see Table 5 on page For delay volume optimization see the Rapid Resolution System manual. Buffer salt of aqueous buffers may precipitate in residual isopropanol. Capillaries and filter may be cloged by precipitating salt. If possible apply solvent conditions that will decrease the gas solubility over time for example, warming up the solvents. The seal wash option should be used when buffer solutions with concentrations of 0.
Damaged plungers cause micro leaks and will decrease the lifetime of the seals. The default compressibility settings are set accordingly. Details about setting up module specific parameters can be found in the Instant Pilot online help. Overview Most of these panels can be accessed in two different ways: Pulling down the Instrumentmenu or left-clicking on the icon the GUI. Open it either from the Instrument menu or by left-clicking the pump icon in the graphical user interface GUI.
Total flow rate of the pump. The stop time of the pump usually controls the run time of the whole LC system. Use no limit to stop the run manually useful for method development. Time between the end of a run and the start of the next. Used for column equilibration after a gradient. Max must be bigger than Min! Set max pressure to the maximum operating pressure of your column. A min pressure setting of e. This channel should be used for the aqueous phase water.
Select the solvent you are using in the respective solvent channel from the drop-down list. Free text field for a description of the solvent. This description will show up in method printouts, etc. Use the timetable to build solvent gradients, flow gradients, or combinations of both.
Gradients are always linear. Use multiple timetable entries to mimic exponential or parabolic gradients. Pump Control The pump control panel is used to turn the pump on and off, operate the optional seal wash pump and define an error method. Upon initialization, the pump ignores the Maximum Flow Gradient value see Table 7 on page This can result in a rapid and uncontrolled pressure increase. The pump group enables you to switch the pump On, Offor to Standby.
In Standby, the pump motor is still energized. When the pump is switched on again, it does not re-initialize. Pump Auxiliary Parameters The parameters in this panel are pre-set to fit most applications.
Adjustments should only be made when required. With this parameter flow rate changes can be ramped up and down slowly to avoid pressure shocks to the column. The flow is shut off immediately when the pump switched to standby. When the pump is turned On from the Off status, the pump drive initializes, thereby ignoring the maximum flow gradient setting. Depending on system delay volume and flow restriction, the system pressure may rise very quickly to a high value.
To protect your column from damage, it is suggested to open the purge valve during initialization. The volume one pump piston delivers per stroke. Generally, a smaller stroke volume results in lower pump ripple. The Auto setting adjusts the strokes dynamically to the lowest possible value. The strokes can be set individually for pump heads A and B. It is highly recommended to tick the Use enhanced compressibility calibration box. This forces the pump to use either stored solvent compressibility data or user generated compressibility parameters from solvent compressibility calibrations.
For legacy support, the solvent compressibility can still be set manually for each channel when the box is unticked. Solvent percentage for each channel, pump flow and pressure are stored when the respective boxes are ticked. Bottle Filling The pump offers a powerful feature to monitor the liquid level in the solvent bottles. With total bottle volume and initial filling volume set correctly, the pump subtracts the displaced volume continuously from the initial value and reacts before the system runs dry or an analysis is corrupted.
The bottle filling feature will fail if multiple channels are fed from one solvent bottle! After filling the solvent bottles, enter the actual volumes into these boxes. The actual volume must not be larger than the total volume of the bottle. When setting this parameter, consider the size and shape of the solvent vessel and make sure the pump does not draw air when coming close to the limit. When ticked, the pump will turn off before air is aspirated.
However, the residual solvent volume has been calculated for 1 L solvent bottles and may be too small for large bottles or other vessels.
Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions, and not on a predefined time interval.
The early maintenance feedback EMF feature monitors the usage of specific components in the instrument, and provides feedback when the user-settable limits have been exceeded. The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled. Each counter increments with pump use, and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded.
Each counter can be reset to zero after maintenance has been done. Liquimeters The liquimeters display the total volume of solvent pumped by the left and right pump heads since the last reset of the counters. Both liquimeters can be assigned an EMF maximum limit. When the limit is exceeded, the EMF flag in the user interface is displayed. Seal Wear Counters The seal wear counters display a value derived from pressure and flow both contribute to seal wear. The values increment with pump usage until the counters are reset after seal maintenance.
Both seal wear counters can be assigned an EMF maximum limit. The wear of pump components is dependent on the analytical conditions, therefore, the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument. Note the pumped volumes and the seal wear values of both pump heads when symptoms of excessive seal wear are observed. Reset the EMF counters to zero.
The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed at the appropriate time, providing a reminder that maintenance needs to be scheduled. This chapter gives information on how to optimize the performance of the Binary Pump SL under special operational conditions.
Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the Binary Pump SL. This is especially true for aqueous solvents or phosphate buffers pH 4 to 7. The following suggestions will prolong lifetime of the solvent filters and maintain the performance of the Binary Pump SL.
A blocked filter therefore does not necessarily affect the high pressure readings of the pump. The pressure readings cannot be used to check whether the filters are blocked or not. If the solvent cabinet is placed on top of the Binary Pump SL, the filter condition can be checked in the following way: Remove the solvent inlet tube from the inlet port of the solvent selection valve or the adapter at the active inlet valve.
If the filter is in good condition, the solvent will freely drip out of the solvent tube due to hydrostatic pressure. If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube. The Binary Pump SL does not necessarily require degassing. Operational Hints for the Vacuum Degasser If you are using the vacuum degasser for the first time, if the vacuum degasser was switched off for any length of time for example, overnight , or if the vacuum degasser chambers are empty, you have to prime the vacuum degasser before running an analysis.
Alternatively, a syringe can be used to draw the solvent through the empty degasser if the pump does not aspirate the solvent by itself. For more information see the Manual of the Agilent Series vacuum degasser.
Concentrated buffer solutions will reduce the lifetime of the seals and plungers in your Binary Pump SL. The active seal wash option allows to maintain the seal lifetime by flushing the low pressure side of the seals with a wash solvent.
The seal wash option is strongly recommended if buffer concentrations of 0. The active seal wash option kit can be ordered by quoting part number G The seal wash option comprises a peristaltic pump, secondary seals, gaskets, seal keepers and tubing for both pump heads. This mixture prevents bacteria growth in the wash bottle and reduces the surface tension of the water. The operation of the peristaltic pump can be controlled from the data system or the Instant Pilot.
The Binary Pump SL comes with pre-installed seal wash capable support rings. When the user decides to use seal wash it is recommended to replace the secondary seals and gaskets by new ones to ensure tightness.
For information on the installation of the continuous seal wash option refer to Installing the Active Seal Wash Option in the Service Manual. The standard seals for the Binary Pump SL can be used for most applications.
However, normal phase applications for example, hexane are not compatible with the standard seals. They cause extremely high abrasion and significantly shorten seal life time. For the use with normal phase applications special polyethylene pistons seals yellow color , part number pack of 2 are available.
These seals have less abrasion compared to the standard seals. The seal wear-in procedure causes problems to the normal phase seals yellow. They will be destroyed by the procedure. Polyethylene seals have a limited pressure range of 0— bar. When used above bar, their lifetime will be significantly reduced. The low volume mixer is designed for use with the Rapid Resolution LC system in low delay volume mode. This configuration is typically used for 2. The low volume mixer helps mixing gradients starting with a low concentration of organic solvents, which can cause noise on the baseline.
The maximum benefit of the mixer is achieved using the mixer together with FW revisions A. The Binary Pump SL is equipped with a pressure pulsation damper and a static mixer. For applications that require lowest delay volume e. This paragraph shows how to bypass damper and mixer and convert the pump to low delay volume mode.
Configurations where only damper or mixer are disconnected while the other part is still in line are not supported by Agilent Technologies. Tools required. It remains unconnected. Disconnect fitting A from outlet 1 of the mixer. Seal port 1 of the mixer with a plastic blank nut. When a solvent is metered at ambient pressure and compressed to a higher pressure, the volume decreases. This is due to an effect known as solvent compressibility.
Solvent compressibility is a non-linear function of pressure and temperature. It is unique to every solvent. In order to deliver the desired flow accurately at all pressures, Agilent pumps use a compressibility compensation. Usually, an average compressibility value for the solvent is used across the whole pressure range of the pump.
The compressibility of a solvent is determined at different pressures between 0 to bar. The pump uses the obtained non-linear function to select the correct compressibility value for the actual pump pressure. Compressibility data for the most common solvents is readily available in the pump firmware. The compensation algorithm is so powerful that the damper and mixer can be removed from the pump flow path at low flow rate while the pressure ripple and composition ripple remain at low levels.
For method compatibility reasons, the legacy compressibility compensation is still available. Solvent Compressibility Calibration Unlisted or premixed solvents can be calibrated with the Solvent Compressibility Calibration function.
The settings represent average values for aqueous solvents A side and organic solvents B side. Therefore it is always recommended to use the aqueous solvent on the A side of the pump and the organic solvent on the B side.
If the compressibility values for the solvents used differ from the default settings, it is recommended to change the compressibility values accordingly.
Compressibility settings can be optimized by using the values for various solvents described in Table 9 on page If the solvent in use is not listed in the compressibility table, when using premixed solvents and if the default settings are not sufficient for your application, the following procedure can be used to optimize the compressibility settings: 1 Start channel A of the Binary Pump SL with the required flow rate. Use degassed solvent only. Re-zero the integrator as required.
The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition. Status Indicators The pump is provided with two status indicators which indicate the operational state pre-run, run, and error states of the module. Error Messages In the event of an electronic, mechanical or hydraulic failure, the Binary Pump SL generates an error message in the user interface.
For each message, a short description of the failure, a list of probable causes of the problem, and a list of suggested actions to fix the problem are provided see Service Manual. Compressibility Calibration Solvent compressibility is a function of solvent type and pressure. In order to optimize flow accuracy and pressure ripple, the compressibility of the solvent must be considered. The Binary Pump SL firmware contains compressibility parameters for most commonly used solvents.
Elasticity Calibration Various parts in the flow path of the Binary Pump SL have a certain elasticity which needs to be compensated for to obtain the lowest pressure-, flow- and composition ripple possible.
This is done by running an elasticity calibration after maintenance and major repairs. For details see Service Manual. Two status indicators are located on the front of the Binary Pump SL. The lower left indicates the power supply status, the upper right indicates the operational status. The power supply indicator is embedded into the main power switch. When the indicator is illuminated green the power is ON.
The pump is in a not-ready state when it is waiting for a specific condition to be reached or completed for example, immediately after changing a set point , or during initialization.
An error condition indicates the pump has detected an internal problem which compromises correct operation of the pump. Usually, an error condition requires attention e. An error condition always interrupts the analysis. Depending on the user interface, the available tests vary. Some descriptions are only available in the Service Manual.
The Agilent Lab Advisor software is a standalone product that can be used with or without data system. Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet.
This includes diagnostic capabilities, calibration procedures and maintenance routines for all the maintenance routines. In addition, users can generate a status report for each individual LC instrument. The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual.
For details refer to the Agilent Lab Advisor software help files. This manual provides lists with the names of Error Messages, Not Ready messages, and other common issues. Description The pressure test is a quick built-in test designed to demonstrate the leak tightness of the system. The test involves monitoring the flow profile while the pump delivers against a blank nut.
The result is presented as the leak rate of the module and provides information about the leak tightness of the system between the outlet ball valves of the pump and the blank nut. The blank nut can be positioned anywhere between the purge valve of the pump and the detector inlet to pressure test the desired part of the system.
Blank not placed at the outlet of flow cell The applied pressure may cause permanent leaks or bursting of the flow cell. Step 1 The test begins with the initialization of both pump heads.
After initialization, the pump is starting the compression phase and the required flow rate is constantly monitored and adjusted. The pump continues to pump until a system pressure of around bar is reached. Step 2 When the system pressure reaches bar, the pump continues to pump at a flow rate that keeps the pressure constant.
The flow that is needed to keep the pressure constant is directly translated into a leak rate. Positioning the blank nut To test the complete system for pressure tightness, the blank nut should be positioned at the column compartment outlet or the outlet of the last module in front of the detector.
If a specific component is suspected to cause the system leak, place the blank nut immediately in front of the suspected component, and run the pressure test again. If the test passes, the defective component is located behind the blank nut. Confirm the diagnosis by placing the blank nut immediately behind the suspected component.
The diagnosis is confirmed if the test fails. The test should be used when problems with small leaks are suspected, or after maintenance of flow path components e. Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with water before starting to pressurize the system!
Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail! Make sure to release the pressure by opening the purge valve when the test has finished completed. Otherwise the pump may generate an overpressure error. Evaluating the Results The sum of all leaks between the pump and the blank nut will add up to the total leak rate. Note that small leaks may cause the test to fail, but solvent may not be seen leaking from a module.
Please notice the difference between an error in the test and a failure of the test! An error is caused by the abnormal termination during the operation of the test whereas a failure of a test indicates that the test results were not within the specified limits.
Repeat the pressure test. Often it is only a damaged blank nut itself poorly shaped from overtightening that causes the test to fail.
